Journal:

Article Title: Effects of functional Toll-like receptor-4 mutations on the immune response to human and experimental sepsis

doi: 10.1046/j.1365-2567.2003.01674.x

Figure Lengend Snippet: TLR4 mutations and sepsis occurrence in surgical patients

Article Snippet: Mouse model of septic peritonitis C3H/HeN mice expressing wild-type TLR4 and TLR4-deficient C3H/HeJ mice were purchased from Harlan Winkelmann (Borchem, Germany).

Techniques:

Journal:

Article Title: Effects of functional Toll-like receptor-4 mutations on the immune response to human and experimental sepsis

doi: 10.1046/j.1365-2567.2003.01674.x

Figure Lengend Snippet: TLR4 mutations and outcome in sepsis patients

Article Snippet: Mouse model of septic peritonitis C3H/HeN mice expressing wild-type TLR4 and TLR4-deficient C3H/HeJ mice were purchased from Harlan Winkelmann (Borchem, Germany).

Techniques:

Journal:

Article Title: Effects of functional Toll-like receptor-4 mutations on the immune response to human and experimental sepsis

doi: 10.1046/j.1365-2567.2003.01674.x

Figure Lengend Snippet: TLR4 genotype distributions and type of infections in sepsis patients

Article Snippet: Mouse model of septic peritonitis C3H/HeN mice expressing wild-type TLR4 and TLR4-deficient C3H/HeJ mice were purchased from Harlan Winkelmann (Borchem, Germany).

Techniques:

Journal:

Article Title: Effects of functional Toll-like receptor-4 mutations on the immune response to human and experimental sepsis

doi: 10.1046/j.1365-2567.2003.01674.x

Figure Lengend Snippet: Influence of the TLR4(D299G) mutation on serum cytokine levels in sepsis patients. Cytokine levels were determined in serum samples of sepsis patients homozygous for wild-type TLR4 (WT), sepsis patients heterozygous for the TLR4(D299G) mutation (MUT), and healthy individuals (Co). Serum samples of sepsis patients were obtained on d1 of sepsis. Numbers indicate the results of statistical comparisons between the patient groups for each cytokine.

Article Snippet: Mouse model of septic peritonitis C3H/HeN mice expressing wild-type TLR4 and TLR4-deficient C3H/HeJ mice were purchased from Harlan Winkelmann (Borchem, Germany).

Techniques: Mutagenesis

Journal:

Article Title: Effects of functional Toll-like receptor-4 mutations on the immune response to human and experimental sepsis

doi: 10.1046/j.1365-2567.2003.01674.x

Figure Lengend Snippet: Serum cytokine levels during experimental septic peritonitis are not influenced by TLR4-deficiency. Serum samples were obtained before (0 hr) and 12 hr after CASP surgery of wild-type C3H/HeN (TLR4+/+) and TLR4-deficient C3H/HeJ mice (TLR4d/d). Statistical analysis indicated that for each cytokine serum levels were not significantly different between the two experimental groups (both at 0 hr and 12 hr). The differences between the 0 hr and 12 hr time points were statistically significant in all cases (P < 0·05).

Article Snippet: Mouse model of septic peritonitis C3H/HeN mice expressing wild-type TLR4 and TLR4-deficient C3H/HeJ mice were purchased from Harlan Winkelmann (Borchem, Germany).

Techniques:

Journal:

Article Title: Effects of functional Toll-like receptor-4 mutations on the immune response to human and experimental sepsis

doi: 10.1046/j.1365-2567.2003.01674.x

Figure Lengend Snippet: Development of acute kidney injury during experimental septic peritonitis is not affected by TLR4-deficiency. Serum samples were obtained before (0 hr) and 12 hr after CASP surgery of wildtype C3H/HeN (TLR4+/+) and TLR4-deficient C3H/HeJ mice (TLR4d/d). Statistical analysis indicated that serum levels of creatinine and urea nitrogen were not significantly different between the two experimental groups (both at 0 hr and 12 hr). The differences between the 0 hr and 12 hr time points were statistically significant in all cases (P < 0·05).

Article Snippet: Mouse model of septic peritonitis C3H/HeN mice expressing wild-type TLR4 and TLR4-deficient C3H/HeJ mice were purchased from Harlan Winkelmann (Borchem, Germany).

Techniques:

Journal: American Journal of Physiology - Renal Physiology

Article Title: Genetic disruption of Npr1 depletes regulatory T cells and provokes high levels of proinflammatory cytokines and fibrosis in the kidneys of female mutant mice

doi: 10.1152/ajprenal.00621.2018

Figure Lengend Snippet: List of the antibodies used in Western blot analysis

Article Snippet: F : quantitative fluorescence intensity analysis of TLR4 protein expression was done by measuring intracellular fluorescence using MetaMorph software.

Techniques: Western Blot

Journal: American Journal of Physiology - Renal Physiology

Article Title: Genetic disruption of Npr1 depletes regulatory T cells and provokes high levels of proinflammatory cytokines and fibrosis in the kidneys of female mutant mice

doi: 10.1152/ajprenal.00621.2018

Figure Lengend Snippet: Forward and reverse sequences of primers that were used to amplify specific mRNAs in the kidney tissue of natriuretic peptide receptor-A gene-targeted mice

Article Snippet: F : quantitative fluorescence intensity analysis of TLR4 protein expression was done by measuring intracellular fluorescence using MetaMorph software.

Techniques: Sequencing

Journal: American Journal of Physiology - Renal Physiology

Article Title: Genetic disruption of Npr1 depletes regulatory T cells and provokes high levels of proinflammatory cytokines and fibrosis in the kidneys of female mutant mice

doi: 10.1152/ajprenal.00621.2018

Figure Lengend Snippet: Analysis of Toll-like receptors (TLRs) and mammalian target of rapamycin (mTOR) expression in natriuretic peptide receptor-A (Npr1) gene-targeted kidneys in untreated mice and mice treated with rapamycin. A–C: relative mRNA expression of TLR2-, TLR4-, and TLR6-targeted genes normalized to β-actin mRNA in kidney tissues. D: renal expression levels of TLR4 protein were determined by Western blot analysis. E: immunofluorescence of TLR4 in rapamycin-treated and vehicle-treated Npr1 gene-targeted mouse kidneys. A marked increase in TLR4 expression occurred in Npr1 gene knockout kidneys but not in the kidneys of wild-type controls. Rapamycin decreased expression of TLR4 in tubular epithelial cells of kidneys. Red fluorescence indicates TLR4 protein expression; blue fluorescence indicates a nucleus stained with DAPI. F: quantitative fluorescence intensity analysis of TLR4 protein expression was done by measuring intracellular fluorescence using MetaMorph software. G: mTOR protein expression was determined by Western blot analysis. β-Actin was used as an internal control for normalization. Densitometric analyses of the respective bands were done with an Alpha Innotech phosphoimager. Values are expressed as means ± SE; n = 6/group. *P < 0.05, control and Npr1++/++ vs. rapamycin-treated Npr1++/++ mice; **P < 0.01, control Npr1+/+ and Npr1++/++ vs. rapamycin-treated Npr1+/+ and Npr1++/++ mice; ***P < 0.001, control Npr1+/− and Npr1−/− vs. rapamycin-treated Npr1+/−and Npr1−/− mice.

Article Snippet: F : quantitative fluorescence intensity analysis of TLR4 protein expression was done by measuring intracellular fluorescence using MetaMorph software.

Techniques: Expressing, Western Blot, Immunofluorescence, Gene Knockout, Fluorescence, Staining, Software, Control